Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: List of primary antibodies

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Diagnostic Assay, Derivative Assay

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: uDISCO clearance of the intact mouse head depicts the expression of aquaporin 1. a Mouse brain (P60) cleared by uDISCO and immunolabeled for AQP1 (AQP1int, green) reveals the vasculature network in the leptomeninges, including the middle cerebral arteries (MCA, arrows). AQP1 + cells also line the subarachnoid cisterns and the olfactory bulb. b Optical section reveals AQP1 + choroidal epithelial cells and olfactory ensheathing glia cells. c , d Higher magnification images of the areas depicted in b (blue and purple squares) showing AQP1 in the glomerular layer (arrow) and in choroidal epithelial cells (asterisk). e Representative micrograph of a parasagittal section of an adult mouse brain (P90) immunolabeled for AQP1 (AQP1ext, grey). AQP1ext + epithelial cells of the choroid plexus are observed in the fourth ( f ) and in the lateral ventricles ( g ). In contrast, olfactory ensheathing glia cells in the olfactory bulb are not immunolabeled ( h ). i Representative micrograph of a coronal section from adult mouse brain (P90) immunolabeled with AQP1 (AQP1int, grey). j Higher magnification of the depicted area in i (square) shows in detail AQP1int + epithelial cells in the choroid plexus of the lateral ventricles. k Olfactory ensheathing glia cells are also immunoreactive. Dashed line in k depicts the mitral cell layer. l Immunoblotting reveals a band of 35 kDa, corresponding to the glycosylated form of AQP1, detected in the BS, Cb, Ctx, Hip, Hyp and OB, obtained from young adult mice (P30). The non-glycosylated form of AQP1, corresponding to a band of 28 kDa, is detected in choroid plexi and kidney homogenates obtained from young adult mice (P30). The housekeeping protein GAPDH (37 kDa) was used as loading control. Control antigen confirms antibody-epitope specific binding. m Graphic shows the relative AQP1 protein levels, in relation to GAPDH. BS, brain stem; Cb, cerebellum; ChP, choroid plexus; Ctx, cerebral cortex; CPu, caudate putamen; EPL, external plexiform layer; Fi, fimbria; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GL, glomerular layer; Hip, hippocampus; Hyp, hypothalamus; IC, internal capsule; IPL, internal plexiform layer; Kdy, kidney; LV, lateral ventricle; OB, olfactory bulb; PirCtx, piriform cortex, SCh, suprachiasmatic nuclei; Thal, thalamus; WM, white matter; 3V, third ventricle; 4V, fourth ventricle. Scale bars: a , b , e 1 mm; c , i 500 μm; d , 200 μm; f – h , j , k 50 μm

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Expressing, Immunolabeling, Western Blot, Binding Assay

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: AQP1 is expressed in the brain and peripheral vasculature. a Confocal micrograph from an adult mouse brain (P90) immunolabeled for AQP1 (AQP1ext, magenta and AQP1int, green). DAPI nuclear counterstaining (blue). b AQP1ext + blood vessel, located around the ventricles (delimited by the magenta square in a ). c – f Immunoreactive epithelial choroid plexus cells, located in the lateral ventricles, are labeled with both antibodies (high magnification of the area delimited by the green square in a ). g , h Micrographs of mouse kidney show the distribution of AQP1 in the vascular endothelium and proximal tubules. i , j Higher magnification image of a blood vessel immunolabeled for CD31 (green) and AQP1int (magenta) (delimited by square in h ). Asterisk indicates the lumen of a blood vessel and arrows indicate proximal tubules. k , l AQP1 + endothelial cells are also detected in the heart of adult mice. m – o Paraffin sections obtained from adult rat brain show AQP1 immunoreactive blood vessels in the hippocampal fissure and epithelial cells of the choroid plexus located in the third ventricle. Arrows and curved arrowheads indicate arterioles or veins and capillaries or venules, respectively. Straight arrowheads indicate AQP1 − blood vessels. 3V, third ventricle; BV, blood vessel; ChP, choroid plexus; DG, dentate gyrus; LV, lateral ventricle; PT, proximal tubule. Scale bars: a , b and g – j 50 µm; c – f 5 µm; k 1 mm; l 100 µm; m 2 mm; n 500 μm; o 200 μm

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Immunolabeling, Labeling

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: AQP1 and NKCC1 are expressed by the choroidal epithelial cells and in the leptomeningeal vasculature. a – f Confocal micrograph show a leptomeningeal WGA-FITC + (green) labeled vessel immunoreactive for AQP1 (magenta) and NKCC1 (orange) in the adult mouse brain (P90). In b an optical section reveals that AQP1 + /NKCC1 + cells are restricted to the smooth muscle cell layer (arrowheads) and absent in the endothelial cells (curved arrowheads), which are labeled by WGA-FITC. g , h NKCC1 is detected in the choroid plexus epithelia, in ependymal cells and in the molecular layer of the cerebellum, as shown in the micrographs of the fourth ventricle. i Double labeling confirms AQP1 and NKCC1 presence in choroid plexus epithelial cells (higher magnification of the area delimited by the blue square in h ). j , k Brain sections obtained from NKCC1 KO adult mice show no immunoreactivity in the brain parenchyma neither in the choroid plexus. l , m Histological sections immunolabeled with antibodies against AQP1ext (magenta), NKCC1 (yellow) and α-SMA (cyan), reveal AQP1ext + /NKCC1 + /α-SMA + leptomeningeal vessels around the hippocampus and third ventricle. Low magnification micrograph shows DAPI (blue) counterstaining and indicates a leptomeningeal blood vessel (asterisk) closely located to the hippocampal fissure. n – p Higher magnification of an AQP1ext + /NKCC1 + vessel (delimited by the dashed square in j . Arrowheads indicate α-SMA + cells. ( q ) Optical sectioning reveals that both AQP1 and NKCC1 are distributed in the smooth muscle cell layer (arrowheads). r 3D rendering of the leptomeningeal vessel confirms AQP1 and NKCC1 restriction to the smooth muscle cell layer (arrowheads). ChP, choroid plexus; DG, dentate gyrus; DS, dorsal subiculum; GL, granular layer; hif, hippocampal fissure; Mol, molecular layer; SAS, subarachnoid space; 3V, third ventricle, 4V, fourth ventricle. Scale bars: a , i 20 µm; b – f , q , r 10 µm; g , h , j – p 50 µm

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Labeling, Immunolabeling

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: AQP1 and NKCC1 are present in smooth muscle and endothelial cells of the leptomeningeal vasculature. a , b Paraffin sections of adult mouse brain (P90) immunolabeled with anti-AQP1int or anti-NKCC1 (both brown). c Some sections were stained with hematoxylin (HE, pink) and the vascular identity of blood vessels located in the subarachnoid space (cisterna interpendicularis, delimited by square in a , b ) was determined. d , e Consecutive sections show that AQP1int + /NKCC1 + cells are present in the smooth muscle cell layer of arterioles (arrowheads) and in the endothelium of capillaries and venules, respectively (curved arrowheads). f , g Vascular endothelial cells were labeled by lectin (WGA-FITC, green), followed by standard Immunolabeling. DAPI counterstain (blue) reveal the location of the leptomeningeal vessel (asterisk). h – j Higher magnification confocal images show that AQP1 is restricted to tunica media, where AQP1ext + smooth muscle cells, identified by their round soma (arrowheads) are observed, whereas AQP1 is not present in the endothelial cell layer (curved arrowheads). The arrow indicates a leptomeningeal cell, also AQP1ext + . BS, brain stem; Cb, cerebellum; cp, cerebral peduncle; Ctx, cerebral cortex; Hip, hippocampus; Hyp, hypothalamus; OB, olfactory bulb; Pn, pontine nuclei. Scale bars: a , b 2 mm; c – e 100 μm; f – j 50 μm

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Immunolabeling, Staining, Labeling

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: AQP1 and NKCC1 are present in leptomeningeal vascular endothelia of the spinal cord. Micrographs of paraffin sections obtained from the spinal cord of adult mice (P90) and immunolabeled for AQP1 and NKCC1 (brown). AQP1 immunoreactivity is predominantly located in C fibers in the dorsal horns of the spinal cord ( a , arrowheads), whereas NKCC1 is observed throughout the spinal cord grey matter ( d ). b , e High magnification of the area delimited by the blue rectangle in a and d , respectively, show AQP1int + /NKCC1 + leptomeningeal vessels (arrows) in the spinal cord. c , f High magnification micrographs of the area delimited by the green squares in b and e show AQP1int + /NKCC1 + cells in the vascular endothelium, restricted to the subarachnoid space along the spinal cord (curved arrowheads). DRG, dorsal root ganglia; SAS, subarachnoid space. Scale bars: a , d 1 mm; b , e 100 μm; c , f 50 μm

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Immunolabeling

Journal: Fluids and Barriers of the CNS

Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system

doi: 10.1186/s12987-020-0176-z

Figure Lengend Snippet: AQP1 and NKCC1 distribution in the CNS leptomeningeal vasculature. Scheme representing the mouse brain parenchyma, the skull and the meninges, which encompass the brain and also the spinal cord. The meninges are divided into the dura mater and the leptomeninges, corresponding to the arachnoid and pia mater. The brain and spinal parenchyma are separated from the meninges by the basal lamina and the glia limitans. The arachnoid mater forms the outer barrier of the CNS and underneath it lies the subarachnoid space (SAS), which is filled with CSF. Immune cells, namely macrophages and leucocytes, are sparsely present within the SAS, surveilling the healthy CNS. Additionally to its function as route for CSF and immune cells circulation, the SAS encloses the arterial blood supply to the CNS. Prior to entering the CNS parenchyma, leptomeningeal arteries branch and divide into arterioles. Within the parenchyma, penetrating arterioles and veins are tethered by astrocytes with highly polarized AQP4 distribution, a unique feature of the CNS vasculature. Schematic representation of cross sections of the leptomeningeal vasculature denotes AQP1 and NKCC1 expression by smooth muscle cells, which compose the tunica media of arterioles and veins. In contrast, endothelial cells within the tunica intima are devoid of both proteins. Notwithstanding, endothelial cells of capillaries and venules present both AQP1 and NKCC1

Article Snippet: We applied different antibodies against AQP1, recognizing epitopes localized both in the intracellular (rabbit anti-AQP1, Alomone Labs, Jerusalem, Israel and rabbit anti-AQP1 Alpha Diagnostic, San Antonio, TX, USA) and in the extracellular (mouse anti-AQP1, Abcam, Cambridge, UK) domains of the protein.

Techniques: Expressing

Journal: Developmental cell

Article Title: Differential expression of NF2 in neuroepithelial compartments is necessary for mammalian eye development

doi: 10.1016/j.devcel.2017.11.011

Figure Lengend Snippet: (A) Sections of mouse embryonic heads and post-natal eyes (C57BL/6J) were immunostained to identify the cells possess mitotic cell cycle marker pH3 and DNA-labeled with BrdU. Nuclei are visualized by DAPI staining. NR, neural retina; pNR, presumptive neural retina; RPE, retinal pigmented epithelium; pRPE, presumptive retinal pigmented epithelium; CM, ciliary margin; ICM, inner ciliary margin; OCM, outer ciliary margin; CB, ciliary body. (B) Quantification of BrdU-positive cell population in the each optic compartment at indicated ages. Error bars in the graphs represent mean ± SEM (n = 6 from 4 independent litters). (C) Expression of Nf2 in the mouse eyes at the indicated ages was examined by immunostaining. Arrows point the Nf2 immunostaining signals. dOV, dorsal optic vesicle; vOV, ventral optic vesicle; LV, lens vesicle; IS, inner segment; OS, outer segment; dOS, dorsal optic stalk; vOS, ventral optic stalk; PCE, pigmented ciliary epithelium; NPCE, non-pigmented ciliary epithelium. (D) Nf2 distribution in the entire ICM (marked by Cdo, left), proximal ICM (marked by Msx1, center), and distal ICM (marked by Aqp1, right) was determined by co-immunodetection of Nf2 and representative ICM markers. Arrowheads indicate the proximal margins of Nf2 staining signals.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Aquaporin 1 (Aqp1) Novus biological (NB600-749) Rat monoclonal Bromodeoxyuridine (BrdU) Abcam (ab6326) Goat polyclonal Brn3b Santa Cruz (sc31987) Rabbit polyclonal Calbindin Swant (CB38a) Mouse polyclonal Cdo R&D Systems (AF2429) Rabbit polyclonal Glutamine synthase (GS) Sigma (G2781) Guinea-pig polyclonal Hes1 Gift from Dr. Ryuichiro Kageyama (Kyoto University) Rabbit polyclonal Mitf Abcam (ab122982) Goat polyclonal Msx1 R&D Systems (AF5045) Rabbit polyclonal NF2 Sigma (HPA003097) Rabbit monoclonal Notch2 Cell Signaling (#5732) Rabbit polyclonal Otx2 Abgent (AP20865c) Rabbit polyclonal Pals1 Upstate (07-708) Rabbit polyclonal Pax6 Biolegend (PRB-278P) Rabbit polyclonal Protein kinase C-α (PKCα) Santa Cruz (sc208) Rabbit polyclonal Recoverin Chemicon (AB5585) Goat polyclonal Sox2 Santa Cruz (sc17320) Rabbit polyclonal Sox9 Millipore (AB5535) Mouse monoclonal Syntaxin Sigma (S0664) Mouse monoclonal Tuj1 Biolegend (MMS-435P) Sheep polyclonal Vsx2 Abcam (ab16142) Rabbit monoclonal Yap/Taz Cell Signaling (#8418) Rabbit polyclonal phospho-histone H3 (pH3) Millipore (06-570) Rabbit polyclonal β-catenin Cell Signaling (#9562) Chicken polyclonal β-galactosidase Abcam (ab9361) Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins Avertin(2,2,2-Tribromoethanol) Sigma {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 Normal donkey serum Jackson Immunoresearch 017-000-121 Hematoxylin Sigma H9627 Eosin Y solution Sigma 318906 5-Bromo-2′-deoxyuridine Sigma B5002 Penicillin-Streptomycin Gibco 15140122 Normal Rabbit IgG Santa Cruz sc-2027 GenJet TM In Vitro DNA Transfection Reagent (Ver.

Techniques: Marker, Labeling, Staining, Expressing, Immunostaining, Immunodetection

Journal: Developmental cell

Article Title: Differential expression of NF2 in neuroepithelial compartments is necessary for mammalian eye development

doi: 10.1016/j.devcel.2017.11.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Aquaporin 1 (Aqp1) Novus biological (NB600-749) Rat monoclonal Bromodeoxyuridine (BrdU) Abcam (ab6326) Goat polyclonal Brn3b Santa Cruz (sc31987) Rabbit polyclonal Calbindin Swant (CB38a) Mouse polyclonal Cdo R&D Systems (AF2429) Rabbit polyclonal Glutamine synthase (GS) Sigma (G2781) Guinea-pig polyclonal Hes1 Gift from Dr. Ryuichiro Kageyama (Kyoto University) Rabbit polyclonal Mitf Abcam (ab122982) Goat polyclonal Msx1 R&D Systems (AF5045) Rabbit polyclonal NF2 Sigma (HPA003097) Rabbit monoclonal Notch2 Cell Signaling (#5732) Rabbit polyclonal Otx2 Abgent (AP20865c) Rabbit polyclonal Pals1 Upstate (07-708) Rabbit polyclonal Pax6 Biolegend (PRB-278P) Rabbit polyclonal Protein kinase C-α (PKCα) Santa Cruz (sc208) Rabbit polyclonal Recoverin Chemicon (AB5585) Goat polyclonal Sox2 Santa Cruz (sc17320) Rabbit polyclonal Sox9 Millipore (AB5535) Mouse monoclonal Syntaxin Sigma (S0664) Mouse monoclonal Tuj1 Biolegend (MMS-435P) Sheep polyclonal Vsx2 Abcam (ab16142) Rabbit monoclonal Yap/Taz Cell Signaling (#8418) Rabbit polyclonal phospho-histone H3 (pH3) Millipore (06-570) Rabbit polyclonal β-catenin Cell Signaling (#9562) Chicken polyclonal β-galactosidase Abcam (ab9361) Bacterial and Virus Strains Biological Samples Chemicals, Peptides, and Recombinant Proteins Avertin(2,2,2-Tribromoethanol) Sigma {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 Normal donkey serum Jackson Immunoresearch 017-000-121 Hematoxylin Sigma H9627 Eosin Y solution Sigma 318906 5-Bromo-2′-deoxyuridine Sigma B5002 Penicillin-Streptomycin Gibco 15140122 Normal Rabbit IgG Santa Cruz sc-2027 GenJet TM In Vitro DNA Transfection Reagent (Ver.

Techniques: Virus, Recombinant, In Vitro, Transfection, Binding Assay, Sequencing, Plasmid Preparation, Control, Software, Modification, SYBR Green Assay

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: Demographic information and clinical characteristics of the 370 patients

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques:

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: Significant association between AQP1 expression and clinical variables. ( A ) The violin plot revealed the variances in AQP1 expression between neoplastic and normal tissues ( p < 0.001). ( B ) Survival analysis using the K-M curve showed that individuals with elevated AQP1 expression in their tumor tissues had prolonged OS ( p < 0.001). ( C ) Survival analysis using the K-M curve showed that individuals with elevated AQP1 expression in their tumor tissues had prolonged PFS ( p < 0.001). ( D ) The analysis using Pearson’s Chi-square test revealed that the presence of AQP1 in tumors was significantly linked with the classification of histopathology, T stage, stage, progression after treatment, OS censor, and PFS classification. ( E ) The forest plot showed the factors that influenced the OS and PFS of renal cancer patients. Elevated AQP1 expression in renal cancer was identified as a protective factor. K-M , Kaplan-Meier; OS , overall survival; PFS , progression-free survival. (F-I) The model diagnosis revealed excellent accuracy and validity

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Expressing, Histopathology, Biomarker Discovery

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: Differential Expression and ceRNAs of AQP1 in Pan-Cancer context. ( A ) Differences in AQP1 expression between tumor and normal tissues according to the TCGA database. ( B ) The miRNAs predicted by three miRNA database, only 38 miRNAs were intersected by all three database. ( C ) AQP1 with its ceRNAs. Yellow triangles were miRNAs and green nodes were lncRNAs. ( D ) Sankey plot demonstrated the relationship of miRNAs and lncRNAs. ( E ) The protein network showed the proteins that coexpressed or co-effected with AQP1. ( F ) The IHC stainings of HNSC, THCA, CESC, COAD, renal cancers, UCEC, and corresponding normal tissue from the HPA database. Staining scores were consistent with the TCGA database. miRNA , microRNA; ceRNA , competitive endogenous RNA; lncRNA , long noncoding RNA; HNSC , head and neck squamous cell carcinoma; THCA , thyroid carcinoma; CESC , cervical squamous cell carcinoma; COAD , colon adenocarcinoma; UCEC , uterine corpus endometrial carcinoma

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Quantitative Proteomics, Expressing, Staining

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: Univariate analyses of survival outcomes in KIRC and pan-cancer context. ( A ) The K-M survival analysis of KIRC, KIRP, HNSC, LGG, UVM showed the correlation of AQP1 expression and OS, PFS. ( B ) Forest plot displayed the HR and 95% CI for the association of AQP1 expression with OS, PFS, DSS, and DFS in pan-cancer. Cancers where AQP1 levels showed a significant negative correlation with survival outcomes were highlighted in red. Conversely, cancers with significant positive correlations between AQP1 levels and survival outcomes were highlighted in blue. K-M survival analysis , Kaplan-Meier survival analysis; KIRC , kidney renal clear cell carcinoma; KIRP , kidney renal papillary cell carcinoma; HR , hazard ratio; 95% CI , 95% confidence interval

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Expressing

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: The AQP1 expression correlated TMB, MSI, genomic changes and drug sensitivity in pan-cancer analysis. ( A ) Radar chart illustrated the correlation between TMB and AQP1 expression across various cancer types. Each spoke of the radar represented a different type of cancer. The distance from the center indicated the strength and direction of the correlation outward represented a positive correlation, and inward indicated a negative correlation. Asterisks next to cancer types denoted statistical significance. AQP1 expression was negatively linked with TMB in UCEC, stomach adenocarcinoma (STAD), skin cutaneous melanoma (SKCM), pancreatic adenocarcinoma (PAAD), LUSC, HNSC, diffuse large B-cell lymphoma (DLBC), BLCA patients. ( B ) This radar chart illustrated the correlation between MSI and AQP1 expression across various cancer types. The blue line traces the correlation values for each cancer type, creating a profile of how AQP1 expression correlated with MSI across the pan-cancer analysis. AQP1 level was positively linked with MSI in LGG, KIRP, and THYM patients. It was negatively correlated in LUAD, LIHC, PAAD, THCA, STAD, PCPG, UCEC, SKCM, READ, HNSC, COAD, PRAD, CESC, LUSC, BLCA, and SARC patients. ( C ) The copy number alterations of AQP1. ( D ) The alteration frequency of AQP1 across various cancer types. ( E ) The mutation types of AQP1 in pan-cancer context. ( F - G ) The nine drugs whose sensitivity related to AQP1 expression predicted by CellMiner database. ( H ) The correlation between CTRP drugs and AQP1 mRNA expression. ( I ) The drugs whose sensitivity related to AQP1 mRNA expression predicted by CTRP. ( J ) The molecular docking analysis of AQP1 and PI-103. TMB , tumor mutation burden; MSI , microsatellite instability

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Expressing, Mutagenesis

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: AQP1 expression correlated with tumor immunity and enrichment pathways. ( A ) The correlation between AQP1 expression and different types of immune cell infiltration of KIRC and KIRP. ( B ) The heatmap of AQP1 expression and IRGs correlation. The Y-axis displayed the analyzed immune genes, with blue indicating inhibitory genes and red indicating promotive genes. The X-axis represented the cancer type. Asterisks denoted statistical significance. The detailed correlation values were shown in triangles, with blue indicating negative values and red indicating positive values. ( C ) The plots showed the enrichment score in the top 5 ranked list of pathways influenced by AQP1 expression. The top portion of the plot displayed the name of pathways. The middle portion marked the position of genes within each set across the ranked list, while the bottom plot provides the ranked list metric, illustrating the distribution of the metric scores across all ranked genes. IRGs , immune related genes

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Expressing

Journal: Human Genomics

Article Title: The comprehensive potential of AQP1 as a tumor biomarker: evidence from kidney neoplasm cohorts, cell experiments and pan-cancer analysis

doi: 10.1186/s40246-025-00726-9

Figure Lengend Snippet: Knockdown of AQP1 in 786-O cells suppressed cell proliferation, migration and invasion. ( A ) WB validated the stable knockdown of AQP1 in 786-O cells. ( B ) The CCK-8 assay demonstrated reduced cell proliferation in AQP1 knockdown cells. ( C ) The colony formation assay revealed that AQP1 knockdown cells formed less colonies than normal controls. ( D ) The transwell migration assay showed that knockdown of AQP1 suppressed cell migration. ( E ) The transwell invasion assay showed that AQP1 knockdown cells inhibited cell invasion. ( F ) The cell scratch assay revealed that knockdown of AQP1 suppressed wound healing. WB , western blot; CCK-8 , Cell counting kit-8

Article Snippet: Knockdown of AQP1 was validated through western blotting (WB), using a rabbit anti-AQP1 antibody at a 1:6000 dilution (#20333-1-AP, Proteintech, IL, USA).

Techniques: Knockdown, Migration, CCK-8 Assay, Colony Assay, Transwell Migration Assay, Transwell Invasion Assay, Wound Healing Assay, Western Blot, Cell Counting

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: Significant changes of AQPs expression in transcription level between ccRCC and normal kidney tissues (Oncomine).

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: mRNA expressions of AQP1/2/3/4/5/6/7/11 in ccRCC were significantly reduced while the expressions of AQP8/9/12B were significantly elevated. The p-value was set at 0.05. (A) Expression of MIP in KIRC based on Sample types. Expression of (B) AQP1, (C) AQP2, (D) AQP3, (E) AQP4, (F) AQP5, (G) AQP6, (H) AQP7, (I) AQP8, (J) AQP9, (K) AQP10, (L) AQP11, (M) AQP12A, (N) AQP12B in KIRC based on Sample types.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: ( A, C, D, G–I ) AQP0/2/3/6/8/9 proteins were not expressed in ccRCC tissues, whereas their low, medium and high expressions were observed in normal kidney tissues. ( B, E ) Low protein expressions of AQP1/4 were found in ccRCC tissues, while their medium protein expressions were observed in normal kidney tissues. ( F, J, K ) AQP5/10/12A&B proteins were expressed neither in ccRCC nor normal kidney tissues. Republished from https://www.proteinatlas.org/ under a CC BY license, with permission from inger åhlén, original copyright 2021.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques:

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: The figure was visualized using violin plots. mRNA expression levels of AQP1/2/4/6/7/9 were remarkably correlated with patients’ individual cancer stages, while the expression across stages did not significantly differ for AQP0/3/5/8/10/11/12A/12B. The method for differential expression gene analysis was one-way ANOVA, using the pathological stage as the variable for calculating differential expression. The F-value was the test statistic from the F-test. The Pr (>F) was the p-value of the F-test and the p-value < 0.05 was significant.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing, Quantitative Proteomics

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: The correlations of AQPs expression with immune infiltration level in ccRCC.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: High mRNA expression levels of AQP0/8/9 were significantly related to shorter OS of patients with ccRCC while high mRNA expression levels of AQP1/4/7 were obviously associated with longer OS time.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: High expressions of AQP1/7 were associated with a better prognosis while high AQP9 expression was correlated with a poorer prognosis of the DFS in ccRCC patients.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: Univariate analysis of overall survival in 526 ccRCC patients.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Gene Expression

Journal: PLoS ONE

Article Title: Abnormal expression and the significant prognostic value of aquaporins in clear cell renal cell carcinoma

doi: 10.1371/journal.pone.0264553

Figure Lengend Snippet: Multivariate analysis of overall survival in 526 ccRCC patients.

Article Snippet: The following antibodies were used: MIP (Anti-MIP polyclonal antibody, Atlas Antibodies, Cat# HPA014940), AQP1 (Anti-AQP1 antibody, Atlas Antibodies, Cat# HPA019206), AQP2 (Anti-AQP2 polyclonal antibody, Atlas Antibodies, Cat# HPA046834), AQP3 (Anti-AQP3 polyclonal antibody, Atlas Antibodies, Cat# HPA014924), AQP4 (Anti-AQP4 polyclonal antibody, Atlas Antibodies, Cat# HPA014784), AQP5 (Anti-AQP5 polyclonal antibody, Atlas Antibodies, Cat# HPA065008), AQP6 (Anti-AQP6 polyclonal antibody, Atlas Antibodies, Cat# HPA015278), AQP7 (pending analysis), AQP8 (Anti-AQP8 polyclonal antibody, Atlas Antibodies, Cat# HPA046259), AQP9 (Anti-AQP9 Antibody, Atlas Antibodies, Cat# HPA074762), AQP10 (Anti-AQP10 polyclonal antibody, Atlas Antibodies, Cat# HPA065947), AQP11 (pending analysis), AQP12A (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216), AQP12B (Anti-AQP12A polyclonal antibody, Atlas Antibodies, Cat# HPA042216).

Techniques: Gene Expression